Figure 9.

PIN1-GFP and Ara7-GFP internalization in PCs after treatment with BFA. A, 35S:PIN1-GFP was transiently expressed for 24 h in leaf PCs, and then these leaves were treated with DMSO or 100 µm BFA. Red arrowheads represent the PIN1 accumulation in BFA bodies. Bars = 20 µm. B, Quantitative analyses of intracellular accumulation of PIN1 in BFA-treated PCs. Asterisks indicate significant difference from the wild type (WT; P < 0.01 by Student’s t test). Error bars represent se (n = 20). C, Distribution of the endocytic marker Ara7-GFP in wild-type and topp4-1 PCs. The leaves of wild-type and topp4-1 plants labeled with Ara7-GFP treated with DMSO or 100 µm BFA. Red arrowheads represent the Ara7-GFP accumulation in BFA bodies. Bars = 50 µm.