Figure 5.

Effect of FaSnRK2.6 OE and RNAi on the expression of ripening-related genes. FaSnRK2.6-OE and FaSnRK2.6-RNAi fruits were generated as described in “Materials and Methods.” The OE and RNAi constructs were injected into fruits at 18 DPA, and gene expression was examined at 30 and 25 DPA, respectively, using qRT-PCR, with FaACTIN as the internal control. OE-C and RNAi-C denote controls for the OE or RNAi fruit (i.e. fruit transformed with the corresponding empty vector). C4H, Cinnamic acid 4-hydroxylase; DFR, dihydroflavonolreductase; OMT, O-methyltransferase; PL, pectatelyase; PG, polygalacturonase. Values are means + sd of four biological replicates. Asterisks denote significant differences compared with the control sample (i.e. OE-C or RNAi-C) at *P < 0.05 and **P < 0.01, using Student’s t test.