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. 2015 Jan 29;167(3):1117–1135. doi: 10.1104/pp.114.253120

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Figure 5. — Effects of wild-type and mutated forms of WtsE on the expression of five genes associated with phenolic metabolism in maize seedlings. Six-day-old cv Seneca Horizon seedlings were vacuum infiltrated with EcDS (pRK415; 1), EcWtsE-FLAG (pJA017; 2), or the EcDS-expressing mutated forms of FLAG-tagged wtsE: w12 mutant (pJA052; 3), wtsEΔ709-1017 (pDM5109; 4), wtsEΔ2935-3234 (pDM5153; 5), or wtsEΔ3235-3534 (pDM5155; 6). Samples were collected at 4 hai, and the expression of TD (A), 4CL (B), C4H (C), P/AD (D), and PAL (E) was assessed. Shown are average values and se of qRT-PCRs done in triplicate for each of three biological replicates. Each biological replicate is two pooled first true leaves. Significantly different expression levels for a given gene and time point are indicated with letters (ANOVA and Fisher’s lsd, P < 0.05). For PAL, gene expression did not differ significantly among these treatments.