Figure 6.

Effects of wild-type and mutated forms of WtsE on the accumulation of CouTyr. B73 maize seedlings were infiltrated with buffer (B) or with the EcDS carrying empty vector (pLAFR3; 1) or plasmids encoding wild-type wtsE (pJA001; 2), w12 mutant (pDM5175; 3), wtsEΔ709-1017 (pDM5189; 4), wtsEΔ2935-3234 (pDM5184; 5), or wtsEΔ3235-3534 (pDM5182; 6). Samples were collected at 12 and 24 hai, and the accumulation of CouTyr was measured by LC-MS/MS. Data, from two samples each from three independent biological replicates (n = 6), are presented as percentages of CouTyr induced by EcDS carrying wild-type wtsE (pJA001) ± sd. Asterisks indicate statistically significant differences in CouTyr levels compared with pJA001, as determined by two-tailed Student’s t test (*P < 0.01, **P < 0.001).