| Strains |
|
|
| E. coli |
|
|
| MC4100 |
araD139 Δ(argF-lac)205 λ− flbB5301 ptsF25 relA1 rpsL150 deoC1
|
Casadaban (1976) |
| DH5α |
mcrBC+ supE44 ΔlacU169 (f80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1
|
Grant et al. (1990) |
|
Pnss
|
|
|
| DC283 |
A spontaneous nalidixic acid resistant (Nalr) derivative of the wild-type strain SS104 |
Coplin et al. (1986) |
| DM711 |
DC283 hrpJ79::Tn3HoHoI, ampicilln resistant (Apr) |
Frederick et al. (2001) |
| DM5101 |
A wtsE::miniTn5gus derivative of DC283, kanamycin resistant (Kmr) |
Ham et al. (2006) |
| Plasmids |
|
|
| pCPP2156 |
Plasmid encoding hrp type III secretion system gene cluster from D. dadantii, specinomycin resistant |
Ham et al. (1998) |
| pCR4-TOPO |
Vector for TOPO cloning PCR products, Apr, Kmr
|
Invitrogen |
| pDM5109 |
pCR4-TOPO with wtsEΔ709-1017-FLAG and wtsF, Apr, Kmr
|
This study |
| pDM5117 |
pRK415 with wtsEΔ709-1017-FLAG and wtsF, tetracycline resistant (Tcr) |
This study |
| pDM5189 |
pLAFR3 with wtsEΔ709-1017 and wtsF, Tcr
|
This study |
| pDM5141 |
pCR4-TOPO with wtsEΔ2935-3234-FLAG and wtsF, Apr, Kmr
|
This study |
| pDM5153 |
pRK415 with wtsEΔ2935-3234-FLAG and wtsF, Tcr
|
This study |
| pDM5184 |
pLAFR3 with wtsEΔ2935-3234 and wtsF, Tcr
|
This study |
| pDM5143 |
pCR4-TOPO with wtsEΔ3235-3534-FLAG and wtsF, Apr, Kmr
|
This study |
| pDM5155 |
pRK415 with wtsEΔ3235-3534-FLAG and wtsF, Tcr
|
This study |
| pDM5182 |
pLAFR3 with wtsEΔ3235-3534 and wtsF, Tcr
|
This study |
| pJA001 |
pLAFR3 with wtsE-F operon and native promoter, Tcr
|
This study |
| pDM5175 |
pLAFR3 with wtsE-w12 (W694A and W840A) and wtsF, Tcr
|
This study |
| pJA006 |
Plasmid derived from pJH001 encoding WtsF and a C terminally FLAG-tagged WtsE driven by native promoter in pCR4-TOPO vector (Invitrogen), Apr, Kmr
|
This study |
| pJA017 |
pRK415 with wtsE encoding a C-terminal FLAG tag driven by native promoter and wtsF, Tcr
|
This study |
| pJA045 |
pCR4-TOPO with wtsE-w12 encoding a C-terminal FLAG tag and wtsF, Apr, Kmr
|
This study |
| pJA052 |
pRK415 with wtsF and wtsE-w12 encoding a C-terminal FLAG tag, Tcr
|
This study |
| pJH001 |
Plasmid containing wtsE-F operon with native promoter in pCR4-TOPO vector, Apr, Kmr
|
Ham et al. (2008) |
| pJH021 |
Plasmid containing wtsE-F operon with native promoter in pBluescript SK(+) (Stratagene), Apr
|
Ham et al. (2008) |
| pJH055 |
Plasmid containing wtsF and wtsE with w12 mutations in pBluescript SK(+), Apr
|
Ham et al. (2009) |
| pCPP1250 |
Plasmid containing dspA/E-F from E. amylovora in pML123, gentamicin resistant |
Bogdanove et al. (1998) |
| pLAFR3 |
Incompatibility group P cosmid, αlacZ, Tcr
|
Staskawicz et al. (1987) |
| pRK415 |
Incompatibility group P αlacZ, Tcr
|
Keen et al. (1988) |
