Figure 2.

Phenotypes of SlZFP2 overexpression and RNAi lines. Representative transgenic lines from LA1589 (A–G) and cv M82 (H–M) show multiple phenotypes during vegetative growth and fruit development. Overexpression of HA-SlZFP2 in both LA1589 (A–C) and cv M82 (F and G) displayed increased branching, shorter plant stature, and early flowering (A and H) and also led to thinner stems (B) and few roots (C). Overexpression of HA-SlZFP2 in cv M82 severely affected seed development (I–M), whereas the SlZFP2 RNAi line of LA1589 showed severely affected fruit set (E and F) compared with its nontransgenic sibling (D). Fruit set in the RNAi line was rescued by pollination with nontransgenic pollen (G). The fruit images of cv M82 overexpression lines (J–M) were from primary (T0) transgenic plants; others were taken from T2 plants. Nontrans, Nontransgenic siblings; OE, overexpression line. Bars = 1 cm.