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. 2015 Jan 30;167(3):931–949. doi: 10.1104/pp.114.255174

Figure 4.

Figure 4.

SlZFP2 promotes seed germination. A, Seed germination of four HA-SlZFP2 overexpression lines of LA1589 and their nontransgenic siblings (Nontrans). Germination based on radicle emergence was determined on seeds harvested from plants grown in the same seasons. B, Seed germination of three HA-SlZFP2 overexpression lines from cv M82 and their nontransgenic siblings. C, Seed germination of three SlZFP2 RNAi lines from LA1589 and their nontransgenic siblings. D, Seed germination of three SlZFP2 RNAi lines from cv M82 and their nontransgenic siblings. E, ABA sensitivity of three HA-SlZFP2 overexpression lines from LA1589 during seed germination. Seeds harvested from the same batch of plants were germinated on one-half-strength Murashige and Skoog medium with ABA supplemented at different concentrations as indicated. F, Fruits of the sit mutant with or without the HA-SlZFP2 transgene. Except for RNAi seeds from line 209, for which fewer than 50 seeds were assayed, at least 150 seeds were tested in triplicate. Seeds used for germination assays were dried for 5 d at room temperature. Data are means ± sd.