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. 2015 Jan 5;167(3):854–871. doi: 10.1104/pp.114.255430

Figure 7.

Figure 7.

Mycorrhizal phenotype in two allelic RAM1 defective mutants. A, Gifu-129, ram1-1, and ram1-2 were inoculated with R. irregularis for 6 weeks. Scale bars = 50 µm. Fungal structures were stained with Alexa Fluor 488. White arrows indicate arbuscules. B, Mycorrhization rate in Gifu-129 and ram1 alleles. Four biological replicates were used. Error bars represent SD (n = 4). Student’s t test was used between Gifu-129 and the two ram1 alleles. Asterisks indicate P < 0.05. C to F, Mycorrhizal marker gene expression in wild-type and ram1 alleles. Mean values (±sd) of four biological replicates are presented. Student’s t test was used. Asterisks indicate significant difference relative to wild-type Gifu-129. *P < 0.05; **P < 0.01.