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. 2015 Jan 20;167(3):817–832. doi: 10.1104/pp.114.256180

Figure 7.

Figure 7.

GRF5 affects chloroplast and cytokinin marker gene expression during leaf development. Relative qRT-PCR expression levels are shown for wild-type Col-0 and 35S:GRF5 leaves 1 and 2 dissected at 6 DAS until 20 DAS from plants grown in vitro. A, The expression of a proliferation- and expansion-specific gene marks the subsequent phases of leaf development. B, Rosettes of Col-0 (top) and 35S:GRF5 (bottom) plants at 6, 11, and 20 DAS. Arrows and circles indicate leaves 1 and 2 that were harvested for qRT-PCR. C, Relative expression levels of GRF5 and marker genes for chloroplast development and cytokinin signaling. Error bars indicate se (n = 3). The insets show magnifications of the graphs of the transition phase from 8 to 11 DAS.