Figure 8.
Relative amounts of AtALMT1 transcripts that carried different lengths of the 5′ untranslated region. A, Transcripts of AtALMT1 were quantified by quantitative reverse transcription PCR using different primer pairs and the TaqMan probe to quantify TSS1-3 (TSS1 primer pair), TSS2 and TSS3 (TSS2 primer pair), and TSS3 (TSS3 primer pair). CDS, Coding DNA sequence. B, Relative proportions of TSS1, TSS2, and TSS3 transcripts at different time points during treatment with 10 μm Al (pH 5.0) for 24 h.