Figure 4.
JARID1B siRNA produce cell apoptosis in Jeko-1 and HL-60 cells. 1 × 105 cells were seeded into a 96-well culture dishes and treated with JARID1B siRNA in 0, 30, 60, 120 nmol/L for 24 hours. Annexin V-PI staining was performed to determine apoptotic cells population. A. Apoptosis was increased gradually after transfected with siRNA JARID1B in 30, 60, 120 nmol/L for 24 hours respectively in Jeko-1, One-way ANOVA: F = 67.5 P < 0.05. B. Apoptosis was increased gradually after transfected with siRNA JARID1B in 30, 60, 120 nmol/L for 24 hours respectively in HL-60, One-way ANOVA: F = 34.8 P < 0.05. Fluorescence signals from Annenxin V-FITC and from PI are reported on the x-axis and y-axis, respectively. Numbers shown in the four quadrants represent the percentage of viable (lower left), necrotic (upper right), early apoptotic (lower right) and late apoptotic (upper right) cells.