Skip to main content
. 2015 Jan 1;8(1):171–183.

Figure 5.

Figure 5

siRNA JARID1B-induced apoptosis was mitochondria-mediated and caspase dependent. Jeko-1 and HL-60 cells were treated respectively for 24 hours with siRNA JARID1B in 0, 30, 60, 120 nmol/L. After siRNA JARID1B treatment, cytosolic proteins were isolated and separated in 12% SDS gel, transferred onto PVDF membrane and blotted with BCL-2, procaspase-3, C-myc antibodys. The proteins were determined by immunoblotting using appropriate antibody. β-actin was used as a loading control. A siRNA JARID1B breakup BCL-2, pro-caspase-3 and C-myc significantly in Jeko-1 cell. B siRNA JARID1B breakup BCL-2, pro-caspase-3 and C-myc in HL-60 cell.