Table 1.
Parameter values used for the models
| Symbol | Description | Value | Source |
|---|---|---|---|
| k1 | Fixed rate of Notch synthesis | 1.5 (nM·min-1) | evaluated |
| k2 | First-order rate constant for Notch cleavage into NICD upon Delta binding | 3.45 (nM-1 min-1) | [16] |
| k3 | Maximum rate of c-hairy-1 gene transcription | 2.5 (nM-1 min-1) | evaluated |
| k4 | First-order rate constant for c-hairy-1 protein synthesis | 4.5 (min-1) | [20] |
| k5 | Threshold constant for activation of c-hairy-1 gene transcription by nuclear NICD | 15 (nM) | evaluated |
| k6 | Maximum rate of Lfng gene transcription | 1.5 (nM-1 min-1) | evaluated |
| k7 | Threshold constant for activation of Lfng gene transcription by nuclear NICD | 10 (nM) | evaluated |
| k8 | First-order rate constant for Lfng protein synthesis | 0.3 (min-1) | [16] |
| k9 | Threshold constant for inhibition of Notch cleavage into NICD by Lfng protein | 0.5 (nM) | [16] |
| k10 | Fixed rate of miRNA synthesis | 0.4 (nM·min-1) | evaluated |
| k11 | Rate for RISC formation | 1 (nM-1 min-1) | evaluated |
| ka | Association constant for c-hariy-1 protein | 1 (nM-1) | [21] |
| kd | Dissociation constant for c-hariy-1 dimer | 4 (min-1) | evaluated |
| d1 | First-order rate of Notch degradation | 2.82 (min-1) | [16] |
| d2 | First-order rate of NICD degradation | 0.06 (min-1) | evaluated |
| d3 | First-order rate of c-hariy-1 mRNA degradation | 0.029 (min-1) | [8] |
| d4 | First-order rate of c-hariy-1 protein degradation | 0.0315 (min-1) | [8] |
| d5 | First-order rate of Lfng mRNA degradation | 0.03 (min-1) | evaluated |
| d6 | First-order rate of Lfng protein degradation | 0.09 (min-1) | evaluated |
| d7 | First-order rate of miRNA degradation | 0.3 (min-1) | evaluated |
| d8 | First-order rate of RISC degradation | 0.1 (nM-1 min-1) | evaluated |
| d9 | First-order rate of c-hariy-1 dimer degradation | 0.12 (min-1) | evaluated |
| m, n, j | Hill coefficient | 2 | [16] |