Figure 3.

Vaspin-induced eNOS phosphorylation is dependent on protein kinase B (Akt) phosphorylation that are in turn regulated upstream by phosphatidylinositol 3-kinase (PI 3-kinase). (A) Vaspin induced eNOS protein expressing in a time dependent manner. (B, C) Representative Western blots for phospho (p)-eNOS (B), p-Akt (C) from EPCs that were stimulated with vaspin (100 ng/ml) alone, with vaspin following incubation with the PI 3-kinase inhibitor LY-294002 (50 μM), or with vaspin post treatment with the Akt kinase inhibitor GSK690693 (10 μM) for 30 min.