Table 1.
Primary antibodies and subcellular location of antigen
| Antibody | Dilution | Company | Subcellular location |
|---|---|---|---|
| p53 | 1:1500 | Dako Corp., Carpentaria, CA | Nucleus |
| p27 | 1:100 | Santa Cruz Biotechnology, Inc., CA | Nucleus, cytoplasm |
| Androgen receptor | 1:100 | Epitomics, CA | Nucleus |
| E2F transcription factor 1 (E2F1) | 1:200 | Invitrogen, Carlsbad, CA | Nucleus |
| Enhancer of zeste homolog 2 (EZH2) | 1:25 | BD Biosciences Pharmingen, San Diego, CA | Nucleus |
| Survivin | 1:50 | Santa Cruz Biotechnology, Inc., CA | Nucleus, cytoplasm |
| Insulin-like growth factor 2 mRNA binding protein 3 (IMP3) | 1:500 | Dako Corp., Carpentaria, CA | Cytoplasm |
| Fatty acid synthase (FASN) | 1:500 | Novus Biologicals, Littleton, CO | Cytoplasm |
| Tuberous sclerosis gene 1 (TSC1)/Hamartin | 1:150 | Abcam, CA | Cytoplasm |
| MAGEA4 | 1:200 | Abcam, CA | Cytoplasm |
| NY-ESO-1 | 1:500 | Invitrogen, Carlsbad, CA | Cytoplasm |
| 14-3-3σ | 1:50 | Santa Cruz Biotechnology, Inc., CA | Cytoplasm |