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. 2015 Jan 1;8(1):319–327.

Figure 4.

Figure 4

MiR-1179 down-regulated SLIT2 by binding the 3’UTR of SLIT2. A: The binding site of miR-1179 with SLIT2. B: Sequence alignment of human miR-1179 with 3’UTR of SLIT2. Bottom: mutations in the 3’-UTR of SLIT2 in order to create the mutant luciferase reporter construct. C: Cells were co-transfected with miR-1179 mimics or miR-control, renilla luciferase vector pRL-SV40 and SLIT2 3’UTR luciferase reporters for 48 h. Both firefly and Renilla luciferase activities are measured in the same sample. The left panel indicated TE-1 cell line while the right indicated Eca-109 Firefly luciferase signals were normalized with Renilla luciferase signals. *indicates significant difference compared with that of control cells (P < 0.05). All tests were performed in triplicate and presented as mean ± SE.