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Expression levels of mRNA encoding hepatocyte-like cell markers and PAS glycogen staining (100× magnification) during induction of hepatic differentiation. A: AFP mRNA levels. B: ALB mRNA levels. C: CK18 mRNA levels. D: Glycogen staining 7 days after induction (no positive cells). E: Glycogen staining 14days after induction. F: Glycogen staining 21 days after induction; **P < 0.01, ***P < 0.001.
