The annexin V-FITC apoptosis detection kit estimated apoptotic cells percentage. A. The apoptotic cell percentage was measured by flow Cytometer after different treatments in the control, H/R injury, sevoflurane preconditioning (SWOP), 3-MA and 3-MA+SWOP groups. B. The flow cytometric histograms of apoptotic rate was showed for each group. *P < 0.05, control vs. H/R, SWOP, 3-MA and 3-MA+SWOP. #P < 0.05, SWOP, 3-MA and 3-MA+SWOP vs. H/R.