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. 2014 Nov 17;124(12):5275–5290. doi: 10.1172/JCI72124

Figure 4. Expression of EWSAT1 is dependent on EWS-FLI1 and independent of the cell of origin.

Figure 4

(A) RT-PCR for EWS-FLI1 expression in the Ewing sarcoma cell line A673 transduced with a hairpin targeting EWS-FLI1 and the indicated cDNA constructs. EWS-FLI1 expression is relative to HPRT. Error bars represent SD for n = 2. (B) Western blot for EWS-FLI1–expressing cDNA constructs. Asterisk indicates V5-tagged EWS-FLI1. (C) RT-PCR for EWSAT1 expression in shEWS-FLI1 A673 cells transduced with indicated cDNA constructs. EWSAT1 expression is relative to HPRT. Error bars represent SD for n = 2. (D) Western blot analysis for EWS-FLI1 using an antibody against the V5 epitope tag in transduced HeLa and 293FT cells. (E) RT-PCR for EWSAT1 expression in transduced HeLa and 293FT cells expressing ectopic EWS-FLI1. EWSAT1 expression is relative to HPRT expression. Pound signs indicate expression below the threshold for detection. Error bars indicate SD.