Skip to main content
. 2014 Oct 27;124(12):5352–5367. doi: 10.1172/JCI76561

Figure 4. BLIMP-1 is a direct target of miR-23a in CTLs.

Figure 4

(A) Schematic representation of the putative miR-23a binding site within the Prdm1 3′-UTR that is conserved across species. (B) Luciferase assays, in which Jurkat T cells were cotransfected with reporter constructs containing full-length 3′-UTRs of the indicated genes, together with the mock or miR-23a overexpression vector. Graph represents mean ± SEM; n = 5. (C and D) iRFP+ mock and miR-23a decoy–expressing pMel-1 CTLs were sorted for miR-23a target studies. (C) Relative mRNA expression of the predicted miR-23a targets Prdm1, Eomes, and Tbet, as well as other CTL effector molecules. Graph represents mean ± SEM; n = 3. (D) BLIMP-1 protein expression, with relative band intensities normalized to β-actin. Left: Representative Western blot. Right: Pooled data from n = 3 independent experiments. P values in B–D were determined by 2-tailed paired t test.