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. 2014 Nov 17;124(12):5109–5128. doi: 10.1172/JCI75695

Figure 13. miR-200 miRNAs are secreted by human metastatic breast cancer cells and transferred to poorly metastatic cells.

Figure 13

(A) Expression of miR-200s in human poorly metastatic MB-231 cells and highly metastatic CA1a and BPLER cells. (B) miRNA copy number in EVs purified from the supernatants of MB-231, CA1a, and BPLER cells. (C) miRNA and mRNA levels in MB-231 cells, cocultured for 3 days separated by a Transwell with MB-231, CA1a, or BPLER cells in which miR-200 was inhibited or not by TuD. (D) miRNA and mRNA levels in MB-231 cells treated for 3 days with EVs purified from the supernatants of MB-231, CA1a, or BPLER cells. In A–D, miRNAs and mRNAs were quantified by TaqMan and Ssofast Evagreen qRT-PCR, respectively (3 experiments). (E) miRNA levels in the sera of mice uninjected (n = 6) or injected with CA1a (n = 6) or BPLER tumors (n = 8) 12 weeks after implantation, determined using the Firefly Circulating miRNA Assay. *P < 0.05; **P < 0.01, Student’s t test.