Skip to main content
. 2014 Nov 17;124(12):5109–5128. doi: 10.1172/JCI75695

Figure 3. miR-200 miRNAs are transferred across a Transwell or by EVs.

Figure 3

(A) miRNA and mRNA levels in 4TO7 cells, untreated or cocultured for 6 days with 4TO7 or 4T1E cells at a 1:4 ratio separated by Transwell. (B) Luciferase reporter assay of 4TO7 cells, transfected with an empty vector (control) or a miRNA reporter for miR-200c or miR-141, and cocultured with 4TO7 or 4T1E cells at a 1:4 ratio in a Transwell plate for 2 days. Renilla luciferase activity, normalized to firefly luciferase, is presented as percentage relative to the control 4TO7 coculture. (C) miRNA and mRNA levels in 4TO7 cells untreated or treated for 3 days with EVs from the culture supernatants of 4TO7 or 4T1E cells. (D) Protein expression in 4TO7 cells untreated or treated as in C. In all panels, 3 experiments are represented. *P < 0.05; **P < 0.01, Student’s t test.