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. 2014 Nov 17;124(12):5109–5128. doi: 10.1172/JCI75695

Figure 8. miR-200 overexpression in mammary fat-pad tumors promotes lung colonization by circulating 4TO7 cells.

Figure 8

(A) Schema of in vivo metastasis assay. 4TO7V or 4TO7OE cells were injected into the mammary fat pad of BALB/c mice, and 10 days later, when the tumor diameter approached 9 mm, 4TO7 cells were injected into the tail vein. Macroscopic lung colonies were counted 8 days later. (B) Representative photographs of the lungs. (C) Colony number per lung. Seven mice in each control group did not receive tail-vein injection; 8 mice bearing 4TO7V tumors and 11 mice bearing 4TO7OE tumors were injected with 4TO7 cells by tail vein. *P < 0.05, Student’s t test. (D) Mammary tumor volume as measured by calipers.