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. 2014 Nov 3;124(12):5249–5262. doi: 10.1172/JCI77598

Figure 1. Spastizin localizes to lysosomes through its FYVE domain.

Figure 1

(A and B) HeLa cells transiently expressing HA-spastizin were coimmunostained for HA-epitope (green) and either endogenous (A) LAMP1 or (B) CD63 (red). (C and D) HeLa cells were coimmunostained for endogenous spastizin (green) and either (C) LAMP1 or (D) CD63 (red). (E) HeLa cells were coimmunostained for either EEA1 or CI-MPR (red) with endogenous spastizin (green). (F) Skin fibroblasts derived from a control subject and a patient with SPG15 were coimmunostained for LAMP1 (red) and endogenous spastizin (green). (G) HeLa cells were transfected with wild-type or mutant HA-spastizin constructs as shown and coimmunostained for HA-epitope (green) and LAMP1 (red). Merged images are to the right in A–D and G. Boxed areas in the images in the top rows are enlarged in the bottom rows in A–D (original magnification, ×2.5), and boxed areas in the images in the left columns are enlarged in the right columns in E and F (original magnification, ×4). Scale bar: 10 μm.