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. 2014 Nov 3;124(12):5424–5436. doi: 10.1172/JCI76289

Figure 5. TGR5 is dispensable for IL-4–induced M2 ATM polarization in vitro.

Figure 5

(A–E) mRNA levels of M2 genes (A) Arg1, (B) Il10, (C) Ym1, (D) Mgl1, and (E) Retnla in primary BMDMs isolated from Tgr5+/+ or Tgr5–/– mice. BMDMs were stimulated with IL-4 (100 ng/ml) for 24 hours in the absence or presence of the TGR5 agonist INT-777 (30 μM). n = 6 per condition. (F) mRNA levels of Tgr5 in primary BMDMs isolated from Tgr5+/+ or Tgr5–/– mice and stimulated with IL-4 (100 ng/ml) or LPS (10 ng/ml) for 24 hours. n = 6 per condition. Results represent mean ± SEM. ***P < 0.001 vs. genotype; #P < 0.001 vs. control, 1-way ANOVA with Bonferroni post-hoc analysis.