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. 2014 Nov 17;124(12):5159–5174. doi: 10.1172/JCI77484

Figure 3. Increased bioavailability of ROS in Gpx1–/– Apoe–/– mice.

Figure 3

(A) Vascular superoxide production, measured by DHE fluorescence, in Gpx1–/– Apoe–/– mice was higher than in the Gpx1+/+ Apoe–/– mouse medial layer (red fluorescence), but not the endothelium or adventitia. Elastic laminae exhibit green autofluorescence. Scale bar: 5 μm. *P < 0.01. n = 6/group. (B) Aortic superoxide production using lucigenin chemiluminescence was higher in Gpx1–/– Apoe–/– compared with Gpx1+/+ Apoe–/– mice without a contribution by the eNOS inhibitor L-NAME, suggesting minimal contribution from endothelium. *P < 0.01. n = 7/group. (C) Net NO levels were significantly decreased in Gpx1–/– Apoe–/– compared with Gpx1+/+ Apoe–/– mice that underwent BAS using Fe-DETC EPR. L-NAME did not contribute to net NO levels. n = 6/group. (D) Immunoblotting for eNOS protein in aortic lysates revealed no difference in eNOS band intensity normalized to GAPDH control between groups. n = 6–8.