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. 2014 Nov 10;124(12):5305–5316. doi: 10.1172/JCI77440

Figure 3. IL-12 neutralization reduces NKG2C+ subset expansion in HCMV-infected cocultures.

Figure 3

(A) PBMCs were cultured with uninfected or AD169-infected MRC-5 fibroblasts in the presence of neutralizing mAb against IL-12 or isotype control. At the end of the coculture, cells were stained for NKp46, NKG2C, and CD3 and analyzed by flow cytometry. Dot plots were gated on live CD3– cells. Numbers indicate the percentage of NKG2C+ cells among all NKp46+ cells (1 representative donor out of 10 is shown). (B–D) Summary of cocultures with neutralizing mAbs against IL-12, IL-15, and IL-18. Depicted is the fold increase of percentage of NKG2C+ NKp46+CD3– cells in infected versus uninfected cocultures. Wilcoxon matched pairs signed-rank test (B) IL-12 neutralization. **P = 0.0039; n = 10. (C) IL-15 neutralization (n = 5) or (D) IL-18 neutralization (n = 5). (E) ELISA for IL-12p70 with coculture supernatant at the indicated time points. n = 5–7. Error bars indicate ± SEM (B–E).