Skip to main content
. 2014 Nov 10;124(12):5305–5316. doi: 10.1172/JCI77440

Figure 7. Blockade of CD94 or NKG2C reduces expansion of NKG2C+ NK cells in response to HCMV-infected fibroblasts.

Figure 7

(A) Fibroblasts were stained with anti–HLA-E mAb or isotype control at the indicated time points before and after infection. (B) PBMCs were cultured with uninfected or AD169-infected fibroblasts in the presence of anti-CD94 F(ab)2 fragments or anti-NKG2C mAb or respective isotype controls. At the end of the coculture, cells were stained for NKp46, NKG2C, and CD3 and analyzed by flow cytometry. Dot plots were gated on live CD3– cells (1 representative donor out of 4 is depicted). Numbers indicate the percentages of NKG2C+ cells among all NKp46+ cells. (C) Summary of cocultures with anti-CD94 F(ab)2 fragments or (D) anti-NKG2C mAbs (paired t test: *P = 0.0314, n = 3 in C; *P = 0.0311, n = 4 in D; error bars indicate ± SEM) is shown. Plotted is the fold increase of percentage of NKG2C+NKp46+CD3– cells in infected versus uninfected cocultures.