Downregulation of acetylated histone 3 in the spinal cord after complete Freund's adjuvant (CFA) injection into the hind paw (light microscopy).
(A) Change in the 50% paw withdrawal threshold of the left hind paw at different time points after injection. Data are expressed as mean ± SD, n = 8; one-way analysis of variance and Dunnett's test. aP < 0.01, bP < 0.05, vs. baseline.
(B) Immunohistochemical analysis of acetylated histone 3 expression in the spinal dorsal horn after injection. (a) Naive rat; (b) CFA 1 d; (c) CFA 7 d. An acetylated histone 3 positive cell was determined by examining brown granules in the nucleus (diaminobenzidine staining; scale bar: 200 μm).
(C) Quantitative analysis of acetylated histone 3 expression, using integrated absorbance, in the dorsal horn. Data are expressed as mean ± SEM, n = 5; two-way analysis of variance. aP < 0.05, vs. CFA 1 d group. CFA 1 d: Day 1 after CFA hind paw injection; CFA 7 d: day 7 after CFA hind paw injection; N: naive group.