Table 1. Comparison of Cq values and calculated number of CFU.
Cq-values (±SEM) obtained with PMA-qPCR and calculated log values of viable P. aeruginosa biofilm cells (using the equation for the linear trendline), after treatment with colistin (200 µg/ml), levofloxacin (100 µg/ml) or tobramycin (200 µg/ml) for 24 h and incubation with PMA. The difference in the calculated number of viable cells after treatment is significantly different between mono- and multispecies biofilms (p < 0.05).
| Biofilm type | Treatment | Cq-value (±SEM) | Calculated log viable cells/biofilm | Δlog |
|---|---|---|---|---|
| Multispecies | – | 15.13 ± 0.28 | 8.27 | 1.26 |
| Colistin | 7.01 | |||
| 20.39 ± 0.64 | ||||
| Monospecies | – | 15.98 ± 0.35 | 8.07 | 0.53 |
| Colistin | 7.54 | |||
| 18.18 ± 0.23 | ||||
| Multispecies | – | 12.79 ± 0.38 | 8.85 | 0.95 |
| Levofloxacin | 7.90 | |||
| 16.71 ± 0.38 | ||||
| Monospecies | – | 14.80 ± 0.15 | 8.36 | 1.57 |
| Levofloxacin | 6.79 | |||
| 21.29 ± 0.46 | ||||
| Multispecies | – | 15.04 ± 0.44 | 8.30 | −0.05 |
| Tobramycin | 8.35 | |||
| 14.84 ± 0.43 | ||||
| Monospecies | 14.80 ± 0.15 | 8.36 | −0.02 | |
| Tobramycin | 8.38 | |||
| 14.70 ± 0.18 |