Figure 3.
Hypoxic regulation of transcripts by BLMP-1 is HIF independent. (A–I) Total mRNA was collected from wild-type animals and blmp-1(tm548) mutants (A) or from wild-type animals, hif-1(ia4) mutants, and blmp-1(tm548) mutants (B–I) after incubation in normoxia or hypoxia (0.1% O2) for 12 hr. The expression levels of HIF-independent genes F45D3.4 (A), icl-1 (B), F44E5.5 (C), mnk-1 (D), mod-5 (E), and zip-1 (F) and HIF-dependent genes nhr-57 (G), egl-9 (H), and fmo-2 (I) were analyzed by qRT-PCR. Normalized values are the average of at least three biological replicates. Error bars are SEM. **P-value <0.01 and *P-value <0.05. A Student’s t-test was used to determine significance.