Figure 2.
SUMO was sufficient to increase binding of SET-6 to LIN-1 in yeast cells. Associations of SET-6 with LA fusion proteins were monitored using the yeast two-hybrid system. Bars represent the average and standard deviation of LexA-dependent β-galactosidase activity from three independent yeast transformants. Values were normalized by setting the interaction of SET-6 with LA:LIN-1(1–64) to 100. The LA fusion proteins were expressed at similar levels as determined by Western blotting (data not shown).