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. 2015 Jan 7;5(3):407–415. doi: 10.1534/g3.114.015834

Figure 1.

Figure 1

Comparison of Surveyor and T7E1 efficiencies. Electropherograms obtained on three different DNA products (exon 2a, exon 3, and exon 6) treated using either the Surveyor (top two rows) or T7E1 enzyme (bottom two rows). The Surveyor and T7E1 assays were made on a 50% mix of wild-type and 20-bp deletion mutants. For the Surveyor and T7E1 negative controls (co-), we prepared individual reactions using only wild-type or mutant DNA and pooled the products before electrophoresis. Peaks colored in gray correspond to the internal molecular weight standards: the small molecular weight marker measures 15 bp and the large measures 1500 bp. Peaks labeled with an asterisk (*) correspond to uncleaved DNA, peaks labeled • and ⬥ correspond to cleaved DNA heteroduplex.