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. 2015 Jan 7;5(3):407–415. doi: 10.1534/g3.114.015834

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Copyright © 2015 Vouillot et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of Surveyor and T7E1 sensitivity. Electropherograms obtained on exon 3 DNA products after Surveyor or T7E1 digestion. The percentage indicated on the right corresponds to the quantity of mutant DNA in a pool of mutant and wild-type DNA. The Surveyor and T7E1 negative controls (co-) correspond to reactions made using mutant DNA only (100%). Peaks colored in gray correspond to the internal molecular weight standards: the small molecular weight marker measures 15 bp and the large measures 1500 bp. Peaks labeled with an asterisk (*) correspond to uncleaved DNA, peaks labeled • and ⬥ correspond to cleaved DNA heteroduplex.