. 2015 Mar 4;6:94. doi: 10.3389/fimmu.2015.00094

Table 3.

Activation marker expression on unstimulated T cells as predictors of T-cell effector function.

	CD69 MFI on CD3⁺CD69⁺ T cells				CD25 MFI on CD3⁺CD25⁺ T cells
	β	R² (%)	Variables in the model	p value	β	R² (%)	Variables in the model	p value
Anti-CD3-induced T-cell proliferation^a
Model 1	0.0058	29.17	CD69 MFI on CD3⁺CD69⁺ T cells	0.0008	0.0035	15.78	CD25 MFI on CD3⁺CD25⁺ T cells	0.0181
Model 2	0.0063	44.95	Model 1 + BMI	<0.0001	0.0021	36.29	Model 1 + PA, vitamin D, selenium, n-3 PUFA	0.1490
Anti-CD3-induced IL-2 secretion from T cells
Model 1	0.0242	29.02	CD69 MFI on CD3⁺CD69⁺ T cells	0.0010	0.0276	55.29	CD25 MFI on CD3⁺CD25⁺ T cells	<0.0001
Model 2	0.0205	64.19	Model 1 + age, PA, total calories, vitamin D, iron	0.0002	0.0209	74.00	Model 1 + age, PA, total calories, vitamin D, iron	<0.0001
Anti-CD3-induced IFN-γ secretion from T cells
Model 1	0.9298	5.28	CD69 MFI on CD3⁺CD69⁺ T cells	0.1911	1.2020	12.91	CD25 MFI on CD3⁺CD25⁺ T cells	0.0369
Model 2	0.7203	30.93	Model 1 + age, BMI, PA, total calories, vitamin C, D, selenium, zinc, alcohol	0.4823	0.9655	31.97	Model 1 + vitamin C, D, selenium, n-3 PUFA	0.0958

^aT-cell proliferation was evaluated by quantifying tritiated thymidine incorporation following stimulation with anti-CD3 antibodies, and results are reported as a stimulation index. Stimulation index was calculated by dividing the cpm of the anti-CD3-induced T-cell proliferation by unstimulated T cells.