Extended Data Figure 2. Cse^−/− mice are more vulnerable to stress induced by 3-nitropropionic acid.

Wild-type and Cse^−/− male mice at 8 months of age were injected with a single dose of 3-nitropropionic acid (3-NP) (100 mg kg⁻¹), and lysates were prepared 24 h later from the striatum and cortex and analysed for oxidative stress. a, b, Striata (a) and cortex (b) of Cse^−/− mice show elevated protein oxidation as measured by protein carbonylation, which is more pronounced in the striatum. n = 3 (means ± s.e.m.). c, d, Cse^−/− mice also show augmented levels of protein nitration in the striatum (c) and cortex (d) in comparison with wild-type mice. Note the increased basal level of protein oxidation in the Cse^−/− mice.