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. 2015 Mar 5;46:23. doi: 10.1186/s13567-015-0155-6

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© Muñoz-Caro et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Influence of store-operated calcium entry (SOCE) on E. bovis sporozoites-induced NET formation. PMN were treated with the SOCE-inhibitor 2-APB prior to exposure to E. bovis sporozoites. For negative controls, non-treated PMN were used. For positive controls, zymosan stimulation was used. NET formation was quantified based on DNA-derived fluorescence intensities using an automate plate reader.