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. 2015 Mar 5;46:23. doi: 10.1186/s13567-015-0155-6

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© Muñoz-Caro et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of phosphorylated ERK1/2- and p38 MAPK in sporozoite-exposed PMN and influence of their inhibition on parasite-triggered NETosis. Total proteins of sporozoite-exposed PMN and non-exposed controls were analyzed by SDS/PAGE and immunoblot technique using specific antibodies against non-phosphorylated and phosphorylated ERK1/2 (A) and p38 MAPK (B). Respective bands were densitometrically analyzed. Each bar represents the arithmetic means ± SEM of three PMN donors. Differences were regarded as significant at a level of p ≤ 0.05 compared to negative controls. (C) NET formation in presence of ERK 1/2 (UO126) and p38 (SB 203580) MAPK inhibitors. Stimulation with zymosan served as a positive control; PMN in plain medium as a negative control.