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. 2015 Mar 5;46:23. doi: 10.1186/s13567-015-0155-6

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© Muñoz-Caro et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NET formation triggered by E. bovis merozoites I and oocyst stages. (A) Scanning electron microscopy (SEM) analysis of E. bovis merozoite I-exposed PMN. The arrow indicates a merozoite I being trapped in NET structures. (B) SEM analysis of E. bovis oocyst-exposed PMN revealing NET-like, delicate PMN-derived filaroid structures being attached to oocysts as indicated by arrows. (C) Quantification of E. bovis merozoite I-induced NET formation. PMN were exposed to E. bovis merozoites I, zymosan (positive control) or plain medium (negative control). NET formation was estimated by Pico Green-derived fluorescence intensities. Arithmetic means of three PMN donors, minimum and maximum.