Figure 1.

FXYD2 is expressed in subsets of small DRG neurons. (A) Real-time PCR showed that the highest level of FXYD2 mRNA (Fxyd2) in all tested tissues was observed in the DRG, and very low levels were observed in the spinal cord and brain of adult mice. In situ hybridization showed that FXYD2 mRNA was mainly present in small DRG neurons. Scale bar, 50 μm. (B) Quantitative analysis showed that almost all of FXYD2 mRNA-containing neurons were small DRG neurons with cross-neuronal area less than 600 μm². (C) Co-localization of FXYD2 with neuronal markers by in situ hybridization combined with immunostaining or double fluorescent in situ hybridization. Most of FXYD2 mRNA-positive DRG neurons were peripherin-positive neurons and only a few FXYD2-expressing neurons were NF200-positive large neurons. About half of FXYD2 mRNA-positive neurons bound to IB4 and were non-peptidergic neurons. Some FXYD2-expressing neurons contained mRNA encoding Mrgprd or Mrgprb4. FXYD2 mRNA was also present in TH mRNA-positive neurons. Some CGRP-positive peptidergic neurons also contained FXYD2 mRNA. Scale bar, 50 μm. (D) Diagram showing the profile of FXYD2-expressing neurons in subsets of DRG neurons.