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. 2015 Feb 20;25(3):351–369. doi: 10.1038/cr.2015.22

Figure 2.

Figure 2

Zebrafish Δ113p53M/M mutant is more sensitive to γ-irradiation. (A) Diagram showing the Δ113p53 promoter and an 11-bp deletion in the promoter of Δ113p53M/M mutant. TSS: transcription start site of Δ113p53. RE: p53 response element. The numbers indicate the positions in the Δ113p53 promoter. Out of the deleted 11 bp, 8 bp are within the RE3. (B) Western blot analysis of p53 activation and Δ113p53 induction in WT and Δ113p53M/M embryos injected or uninjected with bcl2L mRNA, followed by 16 gray of γ-ray irradiation. (C, D) WT and Δ113p53M/M embryos with or without injection of bcl2L mRNA at 1 dpf were treated with γ-irradiation. The pictures were taken at 5 dpi (C). The average viabilities of embryos with different treatments were taken from three repeats from 1 to 7 dpi as indicated (D). (E) A TUNEL assay was used to examine apoptotic cells in embryos with different treatments at 8 hpi as indicated. Approximately 20 embryos from each treatment were sampled at each time point. (F) Assessment of DNA DSB by a comet assay in embryos with different treatments as indicated at 2 dpi.