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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: J Immunol. 2007 Dec 1;179(11):7385–7396. doi: 10.4049/jimmunol.179.11.7385

Figure 5. Activation induced nuclear translocation of specific NF-κB heterodimers in NK cells.

Figure 5

Supershift analysis with antibodies to p65, p52, p50, Rel-B and c-Rel was performed on nuclear protein extracts of YTS (A, B), NK92 (C, D) and ex-vivo (E) NK cells. NK cells were treated with TNF-α (A, C) or anti-NKp30 (B, D, E) for 30 minutes. Nuclear extracts preincubated with NF-κB specific Abs were radiolabelled with double stranded oligonucleotide NF-κB probe and reaction products were analyzed on 5% non-denaturing polyacrylamide gels. Selected sections of the autoradiogram are shown highlighting the region of NF-κB bound probe and supershifted bands. Representative results of three independent experiments are shown.