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. Author manuscript; available in PMC: 2015 Dec 20.
Published in final edited form as: FEBS Lett. 2014 Nov 21;588(24):4831–4837. doi: 10.1016/j.febslet.2014.11.018

Figure 2. Phosphorylation of c-MET and downstream targets.

Figure 2

HGF, eNK1 monomer, or eNK1 dimer was added to HUVEC cells at concentrations of A) 1 nM, or B) 10 pM for the indicated timepoints. Negative control indicates basal media alone without added c-MET agonist. Western blots of cell lysates analyzed using a phospho-specific antibody (‘p’) indicate activated c-MET, Akt and Erk. Total protein levels of c-MET, Akt and Erk are shown to relate the fraction of phosphorylated protein to total protein between treatment conditions. Tubulin is shown as a control to indicate total protein loaded per gel lane prior to Western blotting. C) HGF, eNK1 monomer, or eNK1 dimer were added to c-MET expressing cells, and activation was quantified using the DiscoveRx PathHunter system, which measures chemiluminescence as a readout. HGF is depicted as filled squares, eNK1 monomer as filled circles and eNK1 dimer by open circles.