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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Microsc Microanal. 2014 Apr 17;20(4):1111–1119. doi: 10.1017/S1431927614000828

Figure 4. FIB-SEM imaging of the three layers of the cardiac outflow tract wall.

Figure 4

FIB-SEM images of representative regions of the OFT wall layers: (A, D) myocardium, (B, E) cardiac jelly, and (C, F) endocardium. Top row (A–C), depicting regions of ~5.5 μm × 8 μm, demonstrate general tissue contrast and highlight prominent differences in cellular organization and cellular versus extracellular composition of the three layers. Bottom row (D–F), showing higher magnification views, highlights organelle contrast and tissue ultrastructure. n, nucleus; m, myofibrils; f, fibers in extracellular matrix; *, mitochondria; arrow, microvilli.