1× | 15 min at 95 °C |
45× | 30 s at 95 °C 30 s at annealing temperature (60 °C unless otherwise noted) Melt curve from 70 to 90 °C |
1× | 15 min at 95 °C |
45× | 30 s at 95 °C 30 s at annealing temperature (60 °C unless otherwise noted) Melt curve from 70 to 90 °C |