Skip to main content
. Author manuscript; available in PMC: 2016 Feb 3.
Published in final edited form as: Cell Metab. 2015 Feb 3;21(2):334–346. doi: 10.1016/j.cmet.2015.01.002

Figure 6. Identification and validation of Agpat5.

Figure 6

(A) Manhattan plot showing the significance (–log10 of p) of all SNPs and plasma insulin levels after 8 weeks of HF/HS feeding in male BXD RI strains. Candidate gene for genome-wide significant loci are indicated above genome-wide significant loci

(B) Plasma insulin levels based on genotype distribution at peak SNP associated with plasma insulin levels on chromosome 9 (rs36804270). Box and whisker plot depicting mean and distribution.

(C) Liver expression QTL (eQTL) for Agpat5 showing (–log10 of p) of all SNPs on chromosome 8 with indicated position of gene (red arrow) reflective of cis eQTL.

(D) Gonadal adipose expression QTL (eQTL) for Agpat5 showing (–log10 of p) of all SNPs on chromosome 8 with indicated position of gene (red arrow) reflective of cis eQTL.

(E) Agpat5 adipose expression based on genotype distribution at peak SNP associated with plasma insulin levels on chromosome 9 (rs36804270). Box and whisker plot depicting mean and distribution.

(F) IP-GTT in C57BL/6J mice fed a high-fat diet for 12 weeks and treated with control ASO or Agpat5 ASO for 8 weeks.

(G) IP-GTT in rats fed a high-fructose diet for 12 weeks and treated with control ASO or Agpat5 ASO for 8 weeks. Asterisk (*) indicates significance of p < 0.05. Error bars represent SEM.