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. 2015 Mar 4;10(3):e0118835. doi: 10.1371/journal.pone.0118835

Fig 3. Yeast two-hybrid analysis for the interaction between SAA3 and LOX-1.

Fig 3

(A) Bait constructs were the GAL4 DNA-binding domain fused to hSAA3 C-terminal 61 mer or mSAA3. Prey was the GAL4-activation domain fused to the human LOX-1 extracellular domain, or the mouse LOX-1 extracellular domain. The left panel shows the growth of transformed yeasts in synthetic dropout medium (SD-2; -Leu, -Trp). The middle panel indicates interactions between SAA3 and LOX-1 constructs (shown in the right panel) in SD-4 (-Leu, -Trp, -His, -Ade) in a series of dilutions (dilution increases from left to right). (B) Schematic representation of hSAA3 and its serial C-terminal deletion mutants. An actual amino acid sequence is also displayed. (C) Bait constructs were the GAL4 DNA-binding domain fused to hSAA3 C-terminal 61 mer (hSAA3), and its 6 (hSAA3–6AA), 12 (hSAA3–12AA), or 18 amino acid deletion mutant (hSAA3–18AA). Prey was the GAL4-activation domain fused to the human LOX-1 extracellular domain. The left panel shows the growth of transformed yeasts in the synthetic dropout medium (SD-2; -Leu, -Trp). The middle panel indicates interactions between SAA3 and LOX-1 constructs (shown in the right panel) in SD-4 (-Leu, -Trp, -His, -Ade) in a series of dilutions (dilution increases from left to right).