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. 2015 Mar 4;10(3):e0118835. doi: 10.1371/journal.pone.0118835

Fig 4. The hSAA3 C-terminal peptide binds LOX-1.

Fig 4

(A) hSAA3 C-terminal 26 mer peptide input vs bound on human LOX-1. Unit, ng/ml. Error bars represent the standard deviation of four independent experiments. Dissociation constant of the synthetic peptide against human LOX-1 was determined as Kd = 99 ± 22 nM. (B) LOX-1, phospho-ERK, and ERK immunoblottings of the control CHO and human LOX-1 expressing CHO cell lysates 10 min after the hSAA3 C-terminal 26 mer peptide (200 ng/ml) or oxLDL (100 μg/ml) stimulation. (C) Phospho-ERK and ERK immunoblottings of LOX-1 expressing the CHO cell lysate with or without the hSAA3 C-terminal 26 mer peptide. (D) Phospho-ERK and ERK immunoblottings of HUVEC cell lysates with or without the hSAA3 C-terminal 26 mer peptide. Densitometry quantification (normalized by without peptide) of the bands marked by arrow heads is shown below (four independent experiments).