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. 2015 Mar 4;11(3):e1004986. doi: 10.1371/journal.pgen.1004986

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© 2015 Catania et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Profile of ChIP-SEQ for CENP-A^Cnp1 [49] and corresponding position on endogenous cc2. (B) ChIP analysis of CENP-A^Cnp1 levels on plasmids containing one copy (p1xLM) or one copy of OP (p1xOP) transformed into wt-CENP-A^Cnp1 or hi-CENP-A^Cnp1. (C) CENP-A^Cnp1 levels on plasmids containing three copies of a 2 kb fragment, either OP or LM, cloned in tandem repeats (p3xOP and p3xLM) and transformed into wild-type (wt-CENP-A^Cnp1) or into cells expressing high levels of CENP-A^Cnp1 (hi-CENP-A^Cnp1). (D) A plasmid containing one copy of the LM fragment adjacent to two copies of OP (direct repeats) was transformed into wt-CENP-A^Cnp1 and hi-CENP-A^Cnp1 and CENP-A^Cnp1 levels analysed by ChIP (n = 3).