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. 2015 Mar 4;11(3):e1004986. doi: 10.1371/journal.pgen.1004986

Fig 5. Tandem copies of the 2 kb LM region adjacent to heterochromatin/outer repeat are sufficient to establish a functional centromere in wild-type cells.

Fig 5

(A) Schematic representation of plasmids containing 5.6 kb of heterochromatin-forming outer repeat element flanking a full length cc2 (pH-cc2) or three tandem repeats of the LM region (pH-3xLM). (B) Establishment assay in wild-type cells. pH-cc2, pH-3xLM and pH-3xOP were transformed in wild-type cells and the percentage of transformants that contained plasmids with centromere function was assessed. White colonies with red sectors indicate formation of functional centromeres (details in M&M). (C) ChIP analysis of CENP-ACnp1, CENP-CCnp3 and CENP-KSim4 levels reported as %IP for endogenous centromeres (cc1/3) and relative to cc1/3 at act1+ and on plasmids (L and M) (n = 3).